ISOLATION AND MOLECULAR DIAGNOSIS OF ACINETOBACTER BAUMANNII STRAINS FROM VARIOUS CLINICAL SAMPLES AND THEIR CULTURAL PROPERTIES
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Objective: For the optimal and accurate isolation of Acinetobacter baumannii bacteria, which are considered bacteria are increasingly serious health problem in the healthcare community, especially immunosuppressed people and residents' patients' hospital, due to its high resistance to antibiotics. Methods: Accordingly, 300 clinical samples were collected from different hospitals in Mosul city from January to June 2023. They were grown on culture media such as MacConkey and Hichrome Agar for isolation purposes, the number and percentage of all isolated strains for each medium were recorded. Molecular screening was also done by recA gene and 16S rRNA to confirm the diagnosis. Morphological and physiological characteristics were observed on other different media. Results: the 130/43.3% of A. baumannii were isolated from MacConkey medium and 119/91.5% were isolated on Hichrome medium. However, the isolation rate decreased to 15/11.5% in the case of molecular diagnosis with conservative recA gene. The highest isolation rate was from wound samples at 8.3%, followed by urine and burns at the same rate of 7.5%, then throat swabs at 6.6%, followed by sputum at 1.4%, while the bacteria were not isolated from blood, CSF and medical equipment. The morphological characteristics of the bacteria varied greatly on many media such as Eosin –methylene blue EMB medium, Mueller medium, and Nutrient Agar medium in addition to the previous media. Novelty: This research proves the molecular diagnostic ability of the recA gene in A. baumannii bacteria. While highlighting the isolation of these bacteria at the same time on a selective diagnostic medium and comparing the data of these results with each other.
T. Muleshkova, I. Bazukyan, K. Papadimitriou, V. Gotcheva, A. Angelov, and S.G. Dimov, "Exploring the multifaceted genus Acinetobacter: the facts, the concerns and the opportunities," Preprints, vol. 070683, 2024, pp. 1-15. [Online]. Available: https://www.preprints.org/manuscript/202407.0683/v1.
M. Eveillard, M. Kempf, O. Belmonte, H. Pailhories, and M.L. Joly-Guillou, "Reservoirs of Acinetobacter baumannii outside the hospital and potential involvement in emerging human community-acquired infections," Int. J. Infect. Dis., vol. 17, no. 10, pp. e802-e805, 2013.
T.A. Gülen, İ. Ayfer, İ. Ödemiş, and Ü. Kayabaş, "Acinetobacter baumannii infections and antibiotic resistance in hospitalized patients in an education and research hospital: a six-year analysis," Flora J. Infect. Dis. Clin. Microbiol., vol. 25, no. 4, pp. 563-571, 2020, doi: 10.5578/flora.69434.
S.M. Hamed, A.F. Hussein, M.H. Al-Agamy, H.H. Radwan, and M.M. Zafer, "Genetic configuration of genomic resistance islands in Acinetobacter baumannii clinical isolates from Egypt," Front. Microbiol., vol. 13, 878912, 2022, pp. 1-16, doi: 10.3389/fmicb.2022.878912.
K. Upmanyu, Q.M.R. Haq, and R. Singh, "Factors mediating Acinetobacter baumannii biofilm formation: opportunities for developing therapeutics," Curr. Res. Microb. Sci., vol. 3, 100131, 2022, pp. 1-14, doi: 10.1016/j.crmicr.2022.100131.
Ciftci, E. Karakece, A. Atasoy, G. Asik, and İ. Ciftci, "Culture media for detection of Acinetobacter baumannii selective media for detection of A. baumannii," J. Microbiol. Exp., vol. 2, no. 3, pp. 1-4, 2015, doi: 10.1007/s10096-011-1237-7.
Yusuf, E. Skiebe, and G. Wilharm, "Evaluation of CHROMagar Acinetobacter and MacConkey media for the recovery of Acinetobacter baumannii from soil samples," Lett. Appl. Microbiol., vol. 76, no. 2, 2023, article ovac051, doi: 10.1093/lambio/ovac051.
A.O. Ajao, G. Robinson, M.S. Lee, T.D. Ranke, R.A. Venezia, J.P. Furuno, et al., "Comparison of culture media for detection of Acinetobacter baumannii in surveillance cultures of critically-ill patients," Eur. J. Clin. Microbiol. Infect. Dis., vol. 30, no. 11, pp. 1425-1430, 2011, doi: 10.1007/s10096-011-1237-7.
Silva, S.D. Costa Junior, J.L. Lima, J.L.B. Farias Filho, I.M. Cavalcanti, and M.A.V. Maciel, "Investigation of the association of virulence genes and biofilm production with infection and bacterial colonization processes in multidrug-resistant Acinetobacter spp.," Anais da Acad. Bras. Ciênc., vol. 93, e20210245, 2021, pp. 1-13, doi: 10.1590/0001-3765202120210245.
T.O. Ajiboye, E. Skiebe, and G. Wilharm, "Contributions of RecA and RecBCD DNA repair pathways to the oxidative stress response and sensitivity of Acinetobacter baumannii to antibiotics," Int. J. Antimicrob. Agents, vol. 52, no. 5, pp. 629-636, 2018, doi: 10.1016/j.ijantimicag.2018.07.022.
C. Ching, M. Brychcy, B. Nguyen, P. Muller, A.R. Pearson, M. Downs, et al., "RecA levels modulate biofilm development in Acinetobacter baumannii," Mol. Microbiol., vol. 121, no. 2, pp. 196-212, 2024, doi: 10.1111/mmi.15188.
H.J. Benson, Microbiological Applications: a Laboratory Manual in General Microbiology, 14th ed. New York, NY, USA: McGraw-Hill, 2017.
J. Aranda, C. Bardina, A. Beceiro, S. Rumbo, M.P. Cabral, J. Barbé, and G. Bou, "Acinetobacter baumannii RecA protein in repair of DNA damage, antimicrobial resistance, general stress response, and virulence," J. Bacteriol., vol. 193, no. 15, pp. 3740-3747, 2011, doi: 10.1128/JB.00389-11.
Z.F. Azeez and W.A.H. Al-Daraghi, "Clinical risk factors for nosocomial infection caused by Acinetobacter baumannii among Iraqi patients suffering from differing burns," Iraqi J. Biotechnol., vol. 17, no. 3, pp. 61-66, 2018.
H. Ara, "Prevalence of virulence genes in Acinetobacter baumannii isolated from clinical samples in Mymensingh Medical College Hospital," Am. J. Surg. Case Rep., vol. 3, no. 4, pp. 61-63, 2022.
N.M. Abdulhussein and M.S. Mahdi, "Molecular identification of virulence factors and genotyping of Acinetobacter baumannii isolated from clinical samples by ERIC-PCR," Iraqi J. Biotechnol., vol. 22, no. 1, pp. 209-2019, 2023.
T. Obenhuber, T.C. Scheier, T. Stutz, M. Hug, D. Fontein, A. Kaiser, et al., "An outbreak of multi-drug-resistant Acinetobacter baumannii on a burns ICU and its control with multi-faceted containment measures," J. Hosp. Infect., vol. 146, pp. 102-108, 2024, doi: 10.1016/j.jhin.2024.01.002.
H.F.A. Hussain, N.A. Jafar, and A.P.D.S.B. Alrifai, "The antibiogram profile of A. baumannii isolation from patients with UTI in Iraq," J. Pharm. Negat. Results, vol. 13, no. 8, pp. 1313-1317, 2022, doi: 10.1016/j.jiph.2018.11.005.
A.N.M. Al-Najim and E.G. Alsammak, "Antibiofilm activity of mersacidin produced by Bacillus sp.-AE against Acinetobacter baumannii and Acinetobacter junii isolated from clinical specimens," Rafidain J. Sci., vol. 33, no. 2, pp. 21-39, 2024.
N.N. Abd-alkader and W.A. Al-Draghi, "Gene expression of biofilm formation lasB and rblB genes of Acinetobacter baumannii isolated from wounds, burns and environmental samples," Iraqi J. Biotechnol., vol. 22, no. 1, pp. 90-99, 2023.
E. Casarotta, E. Bottari, S. Vannicola, R. Giorgetti, R. Domizi, A. Carsetti, et al., "Antibiotic treatment of Acinetobacter baumannii superinfection in patients with SARS-CoV-2 infection admitted to intensive care unit: an observational retrospective study," Front. Med., vol. 3, 910031, 2022, pp. 1-7, doi: 10.3389/fmed.2022.910031.
L. Semenec, A.K. Cain, C.J. Dawson, Q. Liu, H. Dinh, H. Lott, et al., "Cross-protection and cross-feeding between Klebsiella pneumoniae and Acinetobacter baumannii promotes their co-existence," Nat. Commun., vol. 14, 702, 2023, pp. 1-18, doi: 10.1038/s41467-023-36252-2.
C. Bartal, K.V. Rolston, and L. Nesher, "Carbapenem-resistant Acinetobacter baumannii: colonization, infection and current treatment options," Infect. Dis. Ther., vol. 11, no. 2, pp. 683-694, 2022, doi: 10.1007/s40121-022-00597-w.
S. Li, P. Wang, S. Tian, J. Zhang, "Risk factors and cerebrospinal fluid indexes analysis of intracranial infection by Acinetobacter baumannii after neurosurgery," Heliyon, vol. 9, no. 8, 2023, pp. 1-11, doi: 10.1016/j.heliyon.2023.e18525.
A. Gedefie, W. Demsis, M. Ashagrie, Y. Kassa, M. Tesfaye, M. Tilahun, et al., "Acinetobacter baumannii biofilm formation and its role in disease pathogenesis: a review," Infect. Drug Resist., vol. 14, pp. 3711-3719, 2021, doi: 10.2147/IDR.S332051.
S. Azaiez, M. Haenni, A.B. Cheikh, M.S. Chalbi, A. Messaoudi, L. Tilouch, et al., "Healthcare equipment and personnel reservoirs of carbapenem-resistant Acinetobacter baumannii epidemic clones in intensive care units in a Tunisian hospital," Microorganisms, vol. 11, no. 11, pp. 1-15, 2023, doi: 10.3390/microorganisms11112637.
M. Asif, I.A. Alvi, and S.U. Rehman, "Insight into Acinetobacter baumannii: pathogenesis, global resistance, mechanisms of resistance, treatment options, and alternative modalities," Infect. Drug Resist., vol. 21, pp. 1249-1260, 2018, doi: 10.2147/IDR.S166750.
N.H. Ahmad and G.A. Mohammad, "Identification of Acinetobacter baumannii and determination of MDR and XDR strains," Baghdad Sci. J., vol. 17, no. 3, pp. 0726-0726, 2020.
E. Dahdouh, M. Hajjar, M. Suarez, Z. Daoud, "Acinetobacter baumannii isolated from Lebanese patients: phenotypes and genotypes of resistance, clonality, and determinants of pathogenicity," Front. Cell. Infect. Microbiol., vol. 6, 163, 2016, pp. 1-10, doi: 10.3389/fcimb.2016.00163.
D. Gazel, M.T. Otkun, and A. Akçalı, "In vitro activity of methylene blue and EMB agar on colistin-resistant A. baumannii: An experimental study," J. Med. Microbiol., vol. 68, no. 11, pp. 1607-1613, 2019, doi: 10.1099/jmm.0.001078.
Y. Yakupoğulları, B. Otlu, B. Celik, and H.G. GB, "Performance of MALDI-TOF MS for the identification of gram-negative bacteria grown on Eosin Methylene Blue (EMB) agar: a simple method for improving the effectiveness of identification," Mikrobiyoloji Bülteni, vol. 53, no. 1, pp. 1-11, 2019, doi: 10.5578/mb.67523.
É. Fonseca, F. Freitas, R. Caldart, S. Morgado, A.C. Vicente, "Pyomelanin biosynthetic pathway in pigment-producer strains from the pandemic Acinetobacter baumannii IC-5," Mem. Inst. Oswaldo Cruz, vol. 115, e200371, 2020, pp. 1-6, doi: 10.1590/0074-02760200371.
F. Zhao, H. Liu, Y. Yao, L. Zhang, Z. Zhou, S. Leptihn, et al., "Description of a rare pyomelanin-producing carbapenem-resistant Acinetobacter baumannii strain coharboring chromosomal OXA-23 and NDM-1," Microbiol. Spectr., vol. 10, no. 4, 2022, pp. 1-11, doi: 10.1128/spectrum.02144-22.
S. Saikia, I. Gogoi, A. Oloo, M. Sharma, M. Puzari, and P. Chetia, "Co-production of metallo-β-lactamase and OXA-type β-lactamases in carbapenem-resistant Acinetobacter baumannii clinical isolates in North East India," World J. Microbiol. Biotechnol., vol. 40, no. 167, 2024, doi: 10.1007/s11274-024-03092-7.
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