THE IMPACT OF SLEEP DISORDERS ON CELLULAR IMMUNE REGULATION AMONG UNIVERSITY STUDENTS

Hussein Ali Mohammed AL-Ukaily; Zainab Nashaat Shukur Al-Saadi

doi:10.61796/jmgcb.v2i7.1346

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Hussein Ali Mohammed AL-Ukaily University of Wasit, Iraq
Zainab Nashaat Shukur Al-Saadi University of Wasit, Iraq

Vol. 2 No. 7 (2025): Journal of Medical Genetics and Clinical Biology

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July 2, 2025

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Objective: Sleep is a fundamental biological process that contributes to regulating physiological balance within the body, particularly in maintaining the efficiency of the immune system. Recent literature indicates that sleep disturbances, especially chronic ones, may lead to impaired cellular immune function and increased general inflammatory activity. Based on this hypothesis, this study aimed to evaluate the impact of sleep disturbances on cellular immune regulation in university students by analyzing lymphocyte ratios and concentrations of certain immune cytokines in the blood. Method: The study included (60) male and female students aged (19–24) years, divided into two groups: a group with sleep disturbances (n = 30), identified based on the Pittsburgh Sleep Quality Index questionnaire (PSQI ≥ 6), and a control group (n = 30) with normal sleep patterns. Immune cell ratios (CD3+, CD4+, CD8+, and CD56+) were measured using flow cytometry, while the concentrations of the cytokines IL-6, TNF-α, and IFN-γ were determined using enzyme-linked immunosorbent assay (ELISA). Result: The results showed a significant decrease in the percentages of CD3+, CD4+, and CD56+ cells in the sleep disorder group compared to the control group (p < 0.01), while no significant differences were recorded in the percentages of CD8+ cells. Regarding cytokines, a significant increase in the concentrations of IL-6 and TNF-α was found in those with sleep disorders (p < 0.01), while there was no significant difference in the level of IFN-γ. The study also revealed a moderate positive correlation between PSQI scores and IL-6 concentrations (r = 0.58, p < 0.01), indicating that poor sleep quality is associated with increased immune inflammation. Novelty: These findings highlight the negative biological impact of sleep disturbances on cellular immune function in young university students, and suggest a potential causal relationship between poor sleep quality and the activation of inflammatory pathways.

AL-Ukaily, H. A. M., & Al-Saadi, Z. N. S. (2025). THE IMPACT OF SLEEP DISORDERS ON CELLULAR IMMUNE REGULATION AMONG UNIVERSITY STUDENTS . Journal of Medical Genetics and Clinical Biology, 2(7), 302–312. https://doi.org/10.61796/jmgcb.v2i7.1346

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S. P. Becker, M. A. Jarrett, A. M. Luebbe, A. A. Garner, G. L. Burns, and M. J. Kofler, "Sleep in a large, multi-university sample of college students: sleep problem prevalence, sex differences, and mental health correlates," Sleep Health, vol. 4, no. 2, pp. 174–181, 2018.

H. K. Kang, C. Rhodes, E. Rivers, C. P. Thornton, and T. Rodney, "Prevalence of mental health disorders among undergraduate university students in the United States: A review," J. Psychosoc. Nurs. Ment. Health Serv., vol. 59, no. 2, pp. 17–24, 2021.

B. Sivertsen et al., "Sleep patterns and insomnia in young adults: A national survey of Norwegian university students," J. Sleep Res., vol. 28, no. 2, p. e12790, 2019.

M. Shoqeirat, A. J. Matarneh, M. I. A.-F. Salameh, L. S. M. Alhawari, and A. Algaralleh, "Analysis of sleep disorder prevalence among Jordanian university students: Influences of sociodemographic factors," Pak. J. Life Soc. Sci., vol. 22, no. 1, 2024.

R. De Graaf, M. Tuithof, S. Van Dorsselaer, and M. Ten Have, "Comparing the effects on work performance of mental and physical disorders," Soc. Psychiatry Psychiatr. Epidemiol., vol. 47, pp. 1873–1883, 2012.

World Health Organization, The World Health Report 2003: Shaping the Future. Geneva, Switzerland: WHO, 2003.

L. Besedovsky, T. Lange, and M. Haack, "The sleep-immune crosstalk in health and disease," Physiol. Rev., 2019.

T. Mundula et al., "Chronic systemic low-grade inflammation and modern lifestyle: The dark role of gut microbiota on related diseases with a focus on COVID-19 pandemic," Curr. Med. Chem., vol. 29, no. 33, pp. 5370–5396, 2022.

J. A. Haspel et al., "Perfect timing: circadian rhythms, sleep, and immunity—an NIH workshop summary," JCI Insight, vol. 5, no. 1, p. e131487, 2020.

N. Seyidoglu and C. Aydin, "Insight of the recent perspectives from psychoneuroimmunology: Stress, nutrition and life quality," in PsychoNeuroImmunology: Vol. 1: Integration of Psychology, Neurology, and Immunology, Springer, 2025, pp. 331–353.

N. A. Zubaidi, M. K. AL-Azzawi, M. S. Mahdi, and M. H. Ajmi, "Investigating the influence of biosynthetic silver nanoparticles on kidney and tissue function in mice," Exp. Theor. Nanotechnol., vol. 9, pp. 15–26, 2025.

T. Feuth, "Interactions between sleep, inflammation, immunity and infections: a narrative review," Immun. Inflamm. Dis., vol. 12, no. 10, p. e70046, 2024.

S. Garbarino, P. Lanteri, N. L. Bragazzi, N. Magnavita, and E. Scoditti, "Role of sleep deprivation in immune-related disease risk and outcomes," Commun. Biol., vol. 4, no. 1, p. 1304, 2021.

S. A. Mohammed and N. S. Ahmed, "Self-assessment tool for improving student’s skills regarding the exams in higher education," Sci. J. Univ. Zakho, vol. 11, no. 3, pp. 453–463, 2023.

M. R. Zielinski, J. T. McKenna, and R. W. McCarley, "Functions and mechanisms of sleep," AIMS Neurosci., vol. 3, no. 1, pp. 67–84, 2016.

M. R. Irwin, "Sleep and inflammation: partners in sickness and in health," Nat. Rev. Immunol., vol. 19, no. 11, pp. 702–715, 2019.

L. Besedovsky, T. Lange, and J. Born, "Sleep and immune function," Pflügers Arch. J. Physiol., vol. 463, no. 1, pp. 121–137, 2012.

A. A. Prather, D. Janicki-Deverts, M. H. Hall, and S. Cohen, "Behaviorally assessed sleep and susceptibility to the common cold," Sleep, vol. 38, no. 9, pp. 1353–1359, 2015.

S. Dimitrov et al., "Gαs-coupled receptor signaling and sleep regulate integrin activation of human antigen-specific T cells," J. Exp. Med., vol. 216, no. 3, pp. 517–526, 2019.

R. Ader and N. Cohen, "Psychoneuroimmunology: interactions between the nervous system and the immune system," Lancet, vol. 345, no. 8942, pp. 99–103, 1995.

A. Arjona and D. K. Sarkar, "Circadian oscillations of clock genes, cytolytic factors, and cytokines in rat NK cells," J. Immunol., vol. 174, no. 12, pp. 7618–7624, 2005.

C. Benedict, M. Brytting, A. Markström, J. E. Broman, and H. B. Schiöth, "Acute sleep deprivation has no lasting effects on the human antibody titer response following a novel influenza A H1N1 virus vaccination," BMC Immunol., vol. 13, pp. 1–5, 2012.

K. Spiegel, J. F. Sheridan, and E. Van Cauter, "Effect of sleep deprivation on response to immunization," JAMA, vol. 288, no. 12, pp. 1471–1472, 2002.

S. D. Hershner and R. D. Chervin, "Causes and consequences of sleepiness among college students," Nat. Sci. Sleep, pp. 73–84, 2014.

D. J. Taylor, K. E. Vatthauer, A. D. Bramoweth, C. Ruggero, and B. Roane, "The role of sleep in predicting college academic performance: is it a unique predictor?" Behav. Sleep Med., vol. 11, no. 3, pp. 159–172, 2013.

J. F. Gaultney, "The prevalence of sleep disorders in college students: impact on academic performance," J. Am. Coll. Health, vol. 59, no. 2, pp. 91–97, 2010.

H. A. M. Al-Ukaily, A. A. S. Alqaisi, H. M. Ali, S. A. Kashkul, W. J. Rasheed, and A. J. Abood, "Cytotoxic T lymphocytes’ function in an adenovirus infection," Am. J. Biol. Nat. Sci., vol. 2, no. 4, pp. 81–91, 2025.

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THE IMPACT OF SLEEP DISORDERS ON CELLULAR IMMUNE REGULATION AMONG UNIVERSITY STUDENTS

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