ISOLATION AND MOLECULAR DETECTION OF BACILLUS SUBTILIS ISOLATED FROM BOWEL SYNDROME PATIENTS WITH DEMONSTRATING THE BACTERIA'S ABILITY TO PRODUCE AMYLASE
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Objective: Gram-positive, rod-shaped bacteria and spore-producing species that can endure in highly severe environments are described by the morphology of Bacillus subtilis. Antibacterial medications play a major role in prevention and treatments for bacterial illnesses at both animals and humans, which feeds the global problem of antibiotic overuse. Eventually, this causes pathogens to become more resistant to different antibacterial medications, and super bacteria may even emerge. At the moment, neither the bacteria that are not resistant nor that are susceptible to antimicrobial medications, and the rise in pathogenic strains that are resistant to these medications is a major barrier to the disease's prevention and treatment, making it more challenging to administer. Method: Study include 50 samples were collected for the current investigation. 10(20%) isolates were found to be bacterial after the required testing were completed, and medication sensitivity tests were then carried out. After testing for 8 antibiotics and the result were high resistant against Rifampicin and Ticarcillin and percentage was (80%), (70%) respectively. The bacteria were then subjected to morphological examinations in order to identify the production of the amylase enzyme. Results: A current study show that bacteria was resistant into number of them. According to the study's noteworthy findings, the enzyme was secreted by 4(40%) isolates. Novelty: The bacteria were then subjected to morphological examinations in order to identify the production of the amylase enzyme.
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