SYNTHESIZED NANOPARTICLES FROM PLEUROTUS OSTRAETUS AND STUDY THE EFFECT AGAINST FUSARIUM AND ALTERNARIA
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Objective: The aim of the research is to produce silver nanoparticles (AgNps) using aqueous extract of Pleurotus ostreatus and evaluate the effectiveness of these nanoparticles against fungal isolates. Methods: The investigation was carried out on fungi that were isolated from rotting fruit. Fungi were classified based on their microscopic and morphological characteristics. For the biosynthesis of silver nanoparticles, a quick and eco-friendly method has been created. A practical, environmentally friendly technique producing stabilised silver nanoparticles (AgNps). These were created using an aqueous extract of Pleurotus ostreatus with an average diameter of 30±60 nm and a spherical shape. At 10-3 M of silver nitrate, the reaction is carried out. The produced AgNps were verified by colorimetric transformation from dark brown to grey. Atomic force microscopy (AFM), scanning electron microscope (SEM), and ultraviolet-visible spectroscopy were used to study the properties. Using the well diffusion method, the inhibitory effect of AgNps against fungi was studied by examining the effects of different concentrations (100, 75, 50, and 25). Results: According to the results, AgNps and the extract have strong and comparable antifungal activity. The results showed that the concentration of 100 μg/mL of AgNps was the highest level of inhibition zone identified, with the inhibition zones reaching (23.33 ± 4.41) for Fusarium and at a dose of 25 μg/mL AgNps, the lowest amount of inhibition was found. The zone of inhibition of Alternaria was observed to be 6.00 ± 1.15. The aqueous extract at a concentration of 100 μg/ml showed the largest inhibition zone, with Fusarium inhibition zones reaching 12.33 ± 1.45. At a concentration of 25 μg/ml of aqueous extract, the smallest inhibition zone was observed, with inhibition zones for Alternaria of 4.67 ± 0.33. Novelty: For the biosynthesis of silver nanoparticles, a quick and eco-friendly method has been created. A practical, environmentally friendly technique producing stabilised silver nanoparticles (AgNps).
J. Perfect and A. Casadevall, “Fungal molecular pathogenesis: what can it do and why do we need it?,” in Molecular Principles of Fungal Pathogenesis, J. Heitman, Ed., Washington DC: ASM Press, 2006, pp. 3–11.
K. J. Ryan and C. G. Ray, Sherris Medical Microbiology, 5th ed. USA: McGraw-Hill, 2010.
W. A. Rutala and D. J. Weber, Guideline for Disinfection and Sterilization in Healthcare Facilities. Atlanta, GA, 2008.
N. A. Hanon and F. N. Abd, “The Antimicrobial Activity of Quercus Infectoria Extracts Against Bacteria Isolated from Wounds Infection,” Al-Mustansiriyah J. Sci., vol. 32, no. 1, pp. 1–4, 2021, doi: 10.23851/mjs.v32i1.919.
V. C. Verma, R. N. Kharwar, and A. C. Gange, “Biosynthesis of antimicrobial silver nanoparticles by the endophytic fungus Aspergillus clavatus,” J. Nanomedicine, vol. 5, no. 1, pp. 33–40, 2010.
N. K. Ahmed and O. T. Pirbal, “Nano S_β-Connectedness in Nano Topological Spaces,” Al-Mustansiriyah J. Sci., vol. 34, no. 2, pp. 87–94, 2023, doi: 10.23851/mjs.v34i2.1245.
S. A. A. S. Al Anni, “Isolate and diagnoses of opportunistic fungi from Basrah hospitals with study of effected some disinfectants,” Science College, Basrah University, 1997.
A. M. Awwad, N. M. Salem, and A. O. Abdeen, “Green synthesis of silver nanoparticles using carob leaf extract and its antibacterial activity,” Int. J. Ind. Chem., vol. 4, p. 29, 2013.
N. Hussein and Z. Nabeel, “Antimicrobial Effects of Mentha Pulegium Extract against Staphyloccocus Aureus Bacteria,” Al-Mustansiriyah J. Sci., vol. 29, no. 2, pp. 63–68, 2018, doi: 10.23851/mjs.v29i2.155.
K. S. Prasad, D. Pathak, and A. Patel, “Biogenic synthesis of silver nanoparticles using Nicotiana tobaccum leaf extract and study of their antimicrobial effect,” African J. Biotechnol., vol. 10, no. 41, pp. 8122–8130, 2011.
C. Sia and H. A. L. C. Yim, “Commercial virgin coconut oil: assessment of antimicrobial potential,” Asian J. Food Agro-Industry, vol. 3, pp. 567–579, 2010.
SAS, Statistical Analysis System, User’s Guide, 9.1. Cary, NC, USA: SAS Institute Inc., 2012.
D. R. Reuf, “The epidemiology of hematogenous candidiasis caused by different Candida species,” Clin. Infect. Dis., vol. 24, pp. 1122–1128, 2000.
J. Pulit, M. Banach, R. Szczygłowska, and M. Bryk, “Nanosilver against fungi. Silver nanoparticles as an effective biocidal factor,” Acta Biochem. Pol., vol. 60, no. 4, pp. 795–798, 2013.
J. S. Kim, E. Kuk, K. N. Yu, J. H. Kim, and S. J. Park, “Antimicrobial effects of silver nanoparticles,” Nanomedicine Nanotechnology, Biol. Med., vol. 3, pp. 95–101, 2007.
J. Ibeas and J. Jimenez, “Mitochondrial DNA loss caused by ethanol in Saccharomyces flor yeasts,” Appl. Environ. Microbiol., vol. 63, pp. 7–12, 1997.
H. Alexandre, I. Rousseaux, and C. Charpentier, “Ethanol adaptation mechanisms in Saccharomyces cerevisiae,” Biotechnol. Appl. Biochem., vol. 20, pp. 173–183, 1994.
A. Farrag, M. A. Ismail, K. A. Abdel-Razek, and A. A. Ali, “In vitro antifungal effects of some chemotherapeutic agents against fungi commonly isolated from repeat breeder animals,” J. Basic & Appl. Mycol., vol. 3, pp. 13–19, 2012.
A. H. M. El-Said and G. El-Hady, “Effect of moisture contents on the biodiversity of fungi contaminating Cuminum cyminum and Pimpinella anisum seeds under storage periods and amylolytic activity of fungal isolates,” Int. J. Curr. Microbiol. Appl. Sci., vol. 3, no. 3, pp. 969–991, 2014.
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