Exploring the Ecological Distribution and Antibiotic Resistance Profiles of Klebsiella Species in Diverse Environmental Niches
Downloads
Antimicrobial resistance is a growing problem in bacterial pathogens, particularly in hospital-acquired nosocomial infections. Klebsiella is an important pathogen that causes various infections in hospitalized immunocompromised patients. Therefore, this study aims to fill this knowledge gap and provide insights into the correct detection and antibiotic resistance pattern of these organisms. we will investigate the spread of pneumonia bacteria, Klebsiella, isolate and identify it from different environmental sites, including human, soil, and water, also study the characteristics of antibiotic resistance and find similarities and differences between bacterial isolates. Finally, we detect the associated antibiotics resistance gene (CTX-M), using the genetic RT-PCR method. The study provides valuable information on the prevalence of antibiotic resistant Klebsiella spp. in different clinical and environmental sites, which can help in the development of effective strategies to control the spread of these bacteria. The study found that among all 86 isolated strains of Klebsiella spp. it was found that K. pneumoniae represents the major abundant strain in both clinical and environmental samples by percentages 41.5% and 51.5% respectively. It concerns also the highest ESBL production 71.7%, along with 58% CTX-M gene expression
Riwu, K.H.P., et al., A review: Virulence factors of Klebsiella pneumonia as emerging infection on the food chain. Veterinary World, 2022. 15(9): p. 2172.
Dong, N., et al., Klebsiella species: Taxonomy, hypervirulence and multidrug resistance. EBioMedicine, 2022. 79.
Herridge, W.P., et al., Bacteriophages of Klebsiella spp., their diversity and potential therapeutic uses. Journal of medical microbiology, 2020. 69(2): p. 176.
Janda, J.M. and S.L. Abbott, The changing face of the family Enterobacteriaceae (Order:“Enterobacterales”): New members, taxonomic issues, geographic expansion, and new diseases and disease syndromes. Clinical microbiology reviews, 2021. 34(2): p. 10.1128/cmr. 00174-20.
Ewing, W.H., Enterobacteriaceae; taxonomy and nomenclature. 1966.
Xiong, Y., et al., Genetic diversity and co-prevalence of ESBLs and PMQR genes among plasmid-mediated AmpC β-lactamase-producing Klebsiella pneumoniae isolates causing urinary tract infection. The Journal of Antibiotics, 2021. 74(6): p. 397-406.
Dehshiri, M., et al., The frequency of Klebsiella pneumonia encoding genes for CTX-M, TEM-1 and SHV-1 extended-spectrum beta lactamases enzymes isolated from urinary tract infection. Annals of clinical microbiology and antimicrobials, 2018. 17(1): p. 1-7.
Mir, B.A., S. Rana, and S. Siddesh, Prevalence, antibiotic resistance pattern and identification of extended spectrum beta-lactamase producing Klebsiella pneumoniae from urine samples in a tertiary care hospital. 2020.
Mitra, S.D., et al., Essential oil and phytoconstituent (Linalool) from Homalomena aromatica Schott. rhizomes exhibit antibacterial and synergistic effects with beta-lactam antibiotics against Carbapenem-resistant Enterobacteriaceae (CRE) and Methicillin Resistant S. aureus (MRSA) pathogens. Industrial Crops and Products, 2023. 198: p. 116666.
BINDAYNA, K.M., et al., GENES CONFERRING ANTIMICROBIAL-RESISTANCE AMONG KLEBSIELLA PNEUMONIAE IN THE ARABIAN GULF COUNTRIES: A SYSTEMATIC REVIEW AND META-ANALYSIS. Asian J Pharm Clin Res, 2021. 14(4): p. 90-99.
Navon-Venezia, S., K. Kondratyeva, and A. Carattoli, Klebsiella pneumoniae: a major worldwide source and shuttle for antibiotic resistance. FEMS microbiology reviews, 2017. 41(3): p. 252-275.
Kot, B., et al., Virulence analysis and antibiotic resistance of Klebsiella pneumoniae isolates from hospitalised patients in Poland. Scientific Reports, 2023. 13(1): p. 4448.
Garcia-Bustos, V., M.D. Cabañero-Navalón, and M.S. Lletí, Resistance to beta-lactams in Gram-negative bacilli: relevance and potential therapeutic alternatives. Revista Española de Quimioterapia, 2022. 35(Suppl 2): p. 1.
Hawkey, J., et al., ESBL plasmids in Klebsiella pneumoniae: diversity, transmission and contribution to infection burden in the hospital setting. Genome medicine, 2022. 14(1): p. 1-13.
Livermore, D.M., et al., Carbapenem-resistant Enterobacterales, carbapenem resistant organisms, carbapenemase-producing Enterobacterales, and carbapenemase-producing organisms: terminology past its “sell-by date” in an era of new antibiotics and regional carbapenemase epidemiology. Clinical Infectious Diseases, 2020. 71(7): p. 1776-1782.
O'Riordan, F., et al., Antimicrobial use and antimicrobial resistance in Enterobacterales and Enterococcus faecium: a time series analysis. Journal of Hospital Infection, 2022. 120: p. 57-64.
Choudhury, K., D. Dhar, and A. Bhattacharjee, Molecular characterization of extended spectrum beta lactamases in clinical isolates of Escherichia coli and Klebsiella spp from a tertiary care hospital of South Eastern Assam. Indian Journal of Medical Microbiology, 2022. 40(1): p. 135-137.
Copyright (c) 2024 Dunya Talib Mahdi
This work is licensed under a Creative Commons Attribution 4.0 International License.
